CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. The photograph of pMCs Ig cells shown is representative ( 600). c. The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. Cells were stained with PE conjugated anti CD34 and analyzed by two color FCM. b After a 4 day coculture, EGFP expression levels in CD33+ cells were compared between the three vectors by FCM, and cytospin preparations revealed an immature myeloid cell morphology.
CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation. Coculture at 28 days. Constitutive EGFP expression by pMXs, pMYs, and pMCs in mature myeloid cells (upper panels), and B lymphoid cells (lower panels). The photograph of pMCs Ig cells is representative ( 600). Hairy cell leukemia (HCL) is a rare mature B-cell neoplasm, comprising 2% of lymphoid leukemias, with a predilection for middle-aged and elderly men. Patients commonly present with cytopenias, red pulp involvement of the spleen, and morphologically distinct, circulating cells with cytoplasmic projections and characteristic expression of CD25, CD11c and CD103.
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