Novobiocin Sodium Salt for the production of positively supercoiled plasmid DNA.Inhibitor of bacterial DNA gyrase and eukaryotic DNA topoisomerase.Inhibitor of retrovirus RNA-dependent DNA-polymerase. Novobiocin Sodium Salt is in mammalian cells of the reduction in gene expression regulation (downregulation). Controllable mammals expression system based on new open/close system, switch through the chimeric trans activate protein related compounds coumarin rapidly and sensitive regulation .
The concentration of Novobiocin Sodium Salt antibiotic sweet bean Moore can cause with a chimeric trans activate protein type with dimer formation, and then the driver transcription coding sequence minimum target proteins of promoter of the upper reaches of the operator with λ sequence. In addition, in the antibiotics G-418 existing conditions, pReg neo carrier code of phosphoric acid shift enzyme gene can allow stable and ZhuanRan cells screening and keep the stable value.
The protein expression level can adjust the sweet bean with concentration to control
The protein can and pF12A or pF12K RM Flexi carrier in the promoter region of the control effect happened. Regulation compounds with sweet bean and to instantaneous or stable with excellent pharmacokinetics characteristics in mammalian cells that no significant toxicity, and has no known endogenous combined with target.
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