CD45/CD14（human） has been titrated for optimal performance. Recommended use is 20 uL per test (1x106 cells). recommended for use with either a single or dual laser Flow Cytometer fitted with appropriate acquisition and analysis software.
The flow cytometer must be equipped with a 488 nm laser and must be capable of detecting light scatter (forward and side) and two-color fluorescence with emission detectable in two ranges: 515-545 nm and 562-607 nm.
CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS)-dependent signal transduction, and sCD14 confers LPS sensitivity to cells nonexistent mCD14.
The flow cytometer must cost fitted out with a 488 nm laser and must be confident of discovering brightness level dot (forth and side) and two-color fluorescence with emission detectable in two ranges: 515-545 nm and 562-607 nm.
CD14 is a single-copy gene encoding 2 protein constitutes: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and an monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS)-dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are consociated with rabble-rousing communicable diseases and high gear morbidity in gram-negative shock.
More about: CD45/CD14（human）
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