Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. Monoclonal Anti-Bromodeoxyuridine is designed for studies on cell growth activity by detecting the amount of bromodeoxyuridine incorporated into DNA, western blotting analysis under non-reducing and non-heating conditions and flow cytometry.
BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods include BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA. Antibodies specific for BrdU can then be used to detect the incorporated chemical (see immunohistochemistry), thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues.
However, technical difficulties (see BrdU vs. Tritiated Thymidine) spurred scientists to explore other options, leading us to the more modern technique of BrdU staining. BrdU is a synthetic analogue of Thymidine that is also incorportated into cells during DNA replication, which allows us to successfully identify dividing cells.
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