Insulin from bovine pancreas is used as a growth factor in many mammalian cell culture systems.
Biochem/physiol Actions
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
Reconstitution
Insulin rom bovine pancreas has low solubility at neutral pH. It can be solubilized at 2 mg/ml in dilute acetic or hydrochloric acid, pH 2-3.
More about: Insulin from bovine pancreas
Read more: Biochemical Reagent
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